A12: Investigation of Effects of Salicylic Acid on Mitochondrial TatC Expression in Arabidopsis thaliana

The Twin Arginine Translocation pathway (TAT) is a protein pathway that is fueled only by the proton motive force, can transport fully folded proteins, and is present in bacteria, archaea and eukaryotic organelles such as the chloroplast and mitochondria. It is well known as to the function of this pathway in bacteria and chloroplasts, though it is unknown as to the function of the mitochondrial Tat pathway. In this study, we subjected Arabidopsis thaliana plants to various concentrations of salicylic acid and did a time course study assessing the amount of mtTatC protein present using immunoblotting. We hope to see an increase in mtTatC amount over time and with increasing salicylic acid concentration. Cellular transport is very important for any organism’s cellular functioning and thus this research aids in the understanding of cellular processes that are vital for life.

Authors: Brooke Buckingham

Faculty Advisor: Carole Dabney-Smith, Department of Biochemistry

Graduate Student Advisor: Katie Eudy, Department of Biochemistry

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