The availability of C13 labeled willow tissue (Salix pulchra) has pushed the need for extraction and characterization of polyphenolic compounds within the plant. A method of separation and purification of tannin from the willow plant has been established, but the identity of the extractable non-tannin polyphenols present in willow is not currently known. In order to more fully utilize the isotope labeled tissue, unlabeled control plants were used for pilot studies. The non-tannin polyphenols were extracted and characterized by HPLC/DAD. From the spectral data of the willow extract, we conclude that the non-tannin polyphenols found in willow tissue consist of phenolic acids that elute primarily at the beginning of separation by Sephadex LH-20 column, and flavonoid compounds which elute at the end of the column.
Author: Charles Porter
Faculty Advisors: Drs. Ann Hagerman and Sadeepa Mallikarachchi, Department of Chemistry and Biochemistry
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