My research area is biochemistry about cancer cell. Elevated levels of HMGA1 in cancer cells cause mis-regulation of gene expression and are associated with increased cancer cell proliferation and increased chemotherapy resistance. We used engineered viruses to deliver a decoy hyper binding site (HBS) for HMGA1 to the nucleus of cancer cells. The purpose of this experiment was to determine the effect of virus with HBS DNA on cancer cell. We used restriction enzyme to conduct desired DNA cut. In the process, agarose gel was used to confirm the result. Through ligation and transformation, the cloning of hyper binding site for HMGA into cancer was succeeded. The next step was to conduct virus gene into cancer cell through transfection, it was also called as virus replication. As a result, the virus replication in the cell failed indicating that the virus plasmid needs to be reconstructed and tested again. Therefore, the influence of HBS DNA on cancer cell were uncertain. My intended career is related to analytical chemistry. This experience sparked up my interest in virus and infection. The exploration in this area is significant to me on two levels. I went through practical drills in operating apparatus like centrifuge and PCR instrument and developed an eagerness to expand my knowledge and practice of equipment application. Meanwhile. As samples were measured by microliters and required an extremely high precision, I became increasingly passionate about quantitative research in Analytical chemistry.
Author(s): Shijia Cao, Chemistry Major
Advisor(s): Shuisong Ni, Department of Chemistry and Biochemistry
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