In Miami University’s Bacteriophage class, phages that infect Microbacterium foliorum were isolated. 12 of 20 phages were successfully isolated from organic matter and from that 3 were sequenced and later annotated in MBI 224. The three phages were from three different clusters: EE, EB, and EH. The EE and EB phages were found to be lytic whereas the EH phage is unknown. Tiny Truffula was EE, a more common cluster, with approximately a 17,379 bp genome length. CroZenni and the third phage were from the less common EB and EH clusters, respectively. EB has a genomee of 41,317 bp and EH has one of around 46,728 bp. It was determined that CroZenni was the phage EB by using DOGEMS (Deconvolution of Genomes after En Mass Sequencing), a technique for piecing together complete genomes from a mixture of DNA. DOGEMS was used to try to identify which phage was the EH phage but was never completed. Tiny Truffula had many genes with a known function but the majority of genes from CroZenni had no known function. The DOGEMS method ultimately allowed rare phages to be found.
Authors: Max Brenner, Mackenzie Britton, Alex Flittner, Mikki Fritch, Jonathan Hauman, Meredith Horn, Maddie Killbury, Hannah Latimer, Zach Marsh, Natalie Pantuso, Tyler Pittman, Maria Spaeth, Eian Vargas, Connor Wasmund, Sydney Wissmar, Juliana Zacher, Spencer Zaid, Elle Zenni, Rocco Huston, Avery Imes, Hope Kirby
Advisor(s): Kelly Abshire, Department of Microbiology
Rebecca Balish, Department of Microbiology
Mitchell Balish, Department of Microbiology
Parnell Sheldon, Department of Microbiology
Mariah Squire, Department of Microbiology
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