A69: Synthesis of Electrochemical Biosensor for Fatigue Analysis with Lactate Detection

Blood lactate, a biological analyte, shows a positive correlation with physical exertion and overall fatigue. Blood collection is invasive by nature, therefore we investigated saliva as an alternative biofluid. Studies have shown that salivary lactate levels have a positive correlation with blood lactate levels after exercise. An electrochemical enzymatic biosensor was constructed to quantify lactate concentration. Our sensor was constructed by immobilizing an enzyme (lactate oxidase) on the surface of a screen-printed electrode using gelatin. Results indicate that our desired reaction occurred, however, further iterations are needed to construct an effective salivary lactate sensor.

Author(s): Paige Wagner, Joey Hasak, Olivia Eddy, Marie Burgett

Advisor(s): Shashi Lalvani, Department of Chemical Paper and Biomedical Engineering

Lei Kerr, Department of Chemical, Paper and Biomedical Engineering

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