In this research, we delved into the intricate interactions within the CHIP-mediated ubiquitination pathway, focusing on the interplay among E1-ubiquitin-activating enzyme, E2-ubiquitin-conjugating enzyme (UbcH5b), and E3-ubiquitin ligase (CHIP) with Ubiquitin. The absence of available structures for CHIP in complexes with an E2~ubiquitin conjugate spurred our investigation, aiming to unravel the mechanistic insights and structure of […]