RNA interference (RNAi) and CRISPR/Cas9-genome editing have revolutionized biology by enabling loss-of-function analyses with relatively simple procedures. Although both RNAi and CRISPR/Cas9 lead to functional loss of the targeted gene, these two methods are fundamentally different. RNAi targets mRNA at the post-transcriptional level, leading to the reduction (i.e. knockdown) of the target gene function. On […]
C33: Engineering Auto-catalytic CRISPR-Cas Sensors for Ultra-sensitive Lead Detection
Lead is an industrially useful element, but too much exposure is known to be extremely dangerous to human health. Toxicity and poisoning can result with symptoms such as anemia, kidney and brain damage, and death. A particular concern is lead toxicity in children due to the irreversible neurological damage and developmental defects that can occur. […]