Certain molecules found in cells like Nicotinamide adenine dinucleotide phosphate (NADP), Nicotinamide adenine dinucleotide Hydrogenase (NADH), and flavins exhibit natural fluorescence called autofluorescence. Autofluorescence spectroscopy can be used to identify different metabolic effects caused by these compounds. Phasor analysis is an analytical technique used to assess real time emission signals that could be used to […]
B56: Sensing Cellular Metabolism in Turbid Media Using Spectral Phasor Analysis on Uv-excited Autofluorescence
Reduced nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH) are metabolic cofactors playing significant, distinct roles in cellular metabolism primarily involving cellular respiration and maintaining antioxidant defenses, respectively. Signals from NADH and NADPH are a significant component of cellular autofluorescence and are useful for metabolic sensing. For in vivo sensing of tissues, challenges […]