RNA interference (RNAi) and CRISPR/Cas9-genome editing have revolutionized biology by enabling loss-of-function analyses with relatively simple procedures. Although both RNAi and CRISPR/Cas9 lead to functional loss of the targeted gene, these two methods are fundamentally different. RNAi targets mRNA at the post-transcriptional level, leading to the reduction (i.e. knockdown) of the target gene function. On […]
B05: RNAi of the fmr1 gene of the cricket Acheta domesticus: Impact on male agonistic behavior
Fragile X Syndrome (FXS) is a leading cause of inherited mental disability and autism spectrum disorders. An increased number of CGG repeats (>200) in the fmr1gene results in a loss of the gene’s protein product, Fragile X Mental Retardation Protein (FMRP), resulting in neural deficits and impaired learning and social behaviors. Our goal was to […]