Reduced nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH) are metabolic cofactors playing significant, distinct roles in cellular metabolism primarily involving cellular respiration and maintaining antioxidant defenses, respectively. Signals from NADH and NADPH are a significant component of cellular autofluorescence and are useful for metabolic sensing. For in vivo sensing of tissues, challenges […]
BRII-04: Towards Optics-Based Metabolic Sensing in Tissue
Optical techniques that measure endogenous fluorescence can provide useful information on the status of cellular metabolism, but extensions of these techniques to turbid media (such as tissue) are often complicated by a multitude of emission signals. Phasor analysis is class of analytical approaches used to monitor emission signals that has potential to simplify measurements in […]