RNA interference (RNAi) and CRISPR/Cas9-genome editing have revolutionized biology by enabling loss-of-function analyses with relatively simple procedures. Although both RNAi and CRISPR/Cas9 lead to functional loss of the targeted gene, these two methods are fundamentally different. RNAi targets mRNA at the post-transcriptional level, leading to the reduction (i.e. knockdown) of the target gene function. On […]
BI-03: Examining the effect of Genetic Manipulation on Nerve Remodeling and Eclosion Behavior in Drosophila melanogaster
Abdominal nerves of Drosophila are ensheathed by four layers, the acellular neural lamella (NL), and three glial cell layers. During metamorphosis, a four-day event, five of the eight pairs of abdominal nerves fuse to form the terminal nerve trunk (TNT) structure. During the first day of metamorphosis, the NL is completely degraded. The aim of […]