My project involves using laser autofluorescence spectroscopy paired with spectral phasor analysis, a biophysical technique, to distinguish metabolic changes in yeast cells. I have specifically focused on measuring changes associated with reduced nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH), which are metabolic cofactors associated with cellular respiration and oxidative stress, respectively. Their […]
A24: Developing Non-invasive Real-time Metabolic Monitoring Using Spectral Phasors on Autofluorescence
Spectral phasor analysis of NADH autofluorescence has been shown to be an effective approach to measuring cellular metabolism. Extending this technique to tissue samples offers a novel method for non-invasive, real-time monitoring of healthy and diseased states of tissue. However, complex light interactions within tissue offer a significant barrier to autofluorescence measurements. To characterize the […]
A23: Biomedical Applications of Sensing Multi-State Metabolic Behavior from Cellular Autofluorescence
One way to detect metabolic change in cells is through a method called laser-induced fluorescence spectroscopy which involves exciting the cellular sample using a laser and measuring the spectrum of its autofluorescence emission. In order to detect metabolic change, we monitor autofluorescence emission in real time, and through past studies we are able to quantify […]
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