NADH is an optical metabolic indicator– a molecule we can use to monitor metabolism– because it is naturally occurring, it is involved in energy metabolism, and it fluoresces. NADH fluorescence depends on which protein to which it is bound. UV-excited autofluorescence spectroscopy is a technique used to measure the emission spectrum of excited biological substances. […]
B57: Validating an LED-based Spectrofluorimeter System for Monitoring Cellular Metabolic Response in Tissue-like Environments
Cellular metabolism represents all the series of reactions in a cell that take place in order for the cell to survive. Tissue-like environments simulate a shared network of cells by introducing different forms of turbid media to a cell solution to simulate a real-life environment. Autofluorescence is a method for analyzing metabolic response in biological […]
B56: Sensing Cellular Metabolism in Turbid Media Using Spectral Phasor Analysis on Uv-excited Autofluorescence
Reduced nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH) are metabolic cofactors playing significant, distinct roles in cellular metabolism primarily involving cellular respiration and maintaining antioxidant defenses, respectively. Signals from NADH and NADPH are a significant component of cellular autofluorescence and are useful for metabolic sensing. For in vivo sensing of tissues, challenges […]