{"id":9728,"date":"2024-05-18T12:31:09","date_gmt":"2024-05-18T16:31:09","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=9728"},"modified":"2024-11-07T13:51:19","modified_gmt":"2024-11-07T18:51:19","slug":"b67-plantar-flexor-myofiber-cross-sectional-area-across-a-range-of-physical-inactivity-in-early-postnatal-mice","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/b67-plantar-flexor-myofiber-cross-sectional-area-across-a-range-of-physical-inactivity-in-early-postnatal-mice\/","title":{"rendered":"B67: Plantar flexor myofiber cross-sectional area across a range of physical inactivity in early postnatal mice"},"content":{"rendered":"\n

Physical activity is known to have a positive impact on health while physical inactivity is known to do the opposite. Much less understood is how levels of early life periods of either activity or inactivity, followed by opposite treatment can affect myofibers. 102 mice were separated into 2 cohorts, those completing only 14 days of treatment and those completing treatment and 7 days of post treatment control. The mice in each cohort were seperated into 4 treatment groups of differing activity or inactivity levels. After completing treatment and post treatment, mice were euthanized and muscle samples from the gastrocnemius and plantaris were mounted frozen to be sectioned in ten micron thick sections place on slides. Slides were incubated in antibodies from Developmental Studies Hybridoma Bank specific to Myosin Heavy Chain I, IIa, IIx and IIb with appropriate fluorescent secondary antibodies and imaged on a wide-field fluorescence microscope at the University of Cincinnati Microscopy Core. Sample images were then processed with ImageJ and analyzed through semi-automatic muscle analysis using segmentation of histology (SMASH) for analysis of myofiber cross-sectional area. We found that type I fibers were largely unaffected by changes in physical activity. Increased activity correlated with increased gastrocnemius type II myofiber size while physical inactivity correlated with decreased size of type IIb and IIx myofibers. Our findings allow us to move forward in looking at different muscles of the body, identifying any similarities and differences to our current results, in order to understand better how differing levels of activity and inactivity affect skeletal muscle more broadly.<\/p>\n\n\n\n

Author(s): Waits M., Olivia; Lefkowitz S., Lauren; Kachulkin A., Anthony; Richards E., Katie; Smith D., Austin; Binion A., Rachael; Jevnikar E., Ben; Bagg M., Alexandra; Schaab D., Ty; Sisler M., Maddie; Yeager P., Jackson; Reidy T., Paul<\/p>\n\n\n\n

Advisor(s): Paul Reidy, Kinesiology<\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

Physical activity is known to have a positive impact on health while physical inactivity is known to do the opposite. Much less understood is how levels of early life periods of either activity or inactivity, followed by opposite treatment can affect myofibers. 102 mice were separated into 2 cohorts, those completing only 14 days of […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[1589,1,1592,44],"tags":[86,578,587],"class_list":["post-9728","post","type-post","status-publish","format-standard","hentry","category-2024-posters","category-projects","category-b61-70","category-kinesiology-nutrition-health","tag-health-sciences","tag-kinesiology","tag-physical-activity"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-2wU","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/9728","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=9728"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/9728\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=9728"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=9728"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=9728"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}