{"id":9564,"date":"2024-05-06T20:29:58","date_gmt":"2024-05-07T00:29:58","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=9564"},"modified":"2024-06-16T18:29:36","modified_gmt":"2024-06-16T22:29:36","slug":"c33-distinguishing-the-effect-of-ethanol-and-cyanide-on-cellular-respiration-using-uv-excited-autofluorescence-spectroscopy","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/c33-distinguishing-the-effect-of-ethanol-and-cyanide-on-cellular-respiration-using-uv-excited-autofluorescence-spectroscopy\/","title":{"rendered":"C33: Distinguishing the effect of ethanol and cyanide on cellular respiration using UV-excited autofluorescence spectroscopy"},"content":{"rendered":"\n

NADH is an optical metabolic indicator\u2013 a molecule we can use to monitor metabolism\u2013 because it is naturally occurring, it is involved in energy metabolism, and it fluoresces. NADH fluorescence depends on which protein to which it is bound.<\/p>\n\n\n\n

UV-excited autofluorescence spectroscopy is a technique used to measure the emission spectrum of excited biological substances. This technique is useful in observing metabolic processes. This allows for medical applications, including the recognition of tissue-based conditions, including cancer.<\/p>\n\n\n\n

Phasor analysis is used to quantify the spectrum shape alongside UV-excited autofluorescence spectroscopy to look at cellular NADH and NADPH conformation in baker\u2019s yeast (Saccharomyces cerevisiae) suspensions. For this presentation, we investigated the autofluorescence response to the additions of cyanide and ethanol, which are both respiratory inhibitors.<\/p>\n\n\n\n

Author(s): Dylan Aubel and Quinn Donnelly<\/p>\n\n\n\n

Advisor: Paul Urayama, PhD., Department of Physics<\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

NADH is an optical metabolic indicator\u2013 a molecule we can use to monitor metabolism\u2013 because it is naturally occurring, it is involved in energy metabolism, and it fluoresces. NADH fluorescence depends on which protein to which it is bound. UV-excited autofluorescence spectroscopy is a technique used to measure the emission spectrum of excited biological substances. […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[1589,1,1604,50],"tags":[1103,1682,384,89],"class_list":["post-9564","post","type-post","status-publish","format-standard","hentry","category-2024-posters","category-projects","category-c31-40-2024-posters","category-physics","tag-autofluorescence","tag-biophysics","tag-spectroscopy","tag-stem"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-2ug","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/9564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=9564"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/9564\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=9564"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=9564"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=9564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}