{"id":8488,"date":"2023-04-18T21:35:30","date_gmt":"2023-04-19T01:35:30","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=8488"},"modified":"2023-04-18T21:35:31","modified_gmt":"2023-04-19T01:35:31","slug":"biv-03-effect-of-%ce%bc-opioid-receptor-knockout-on-fear-conditioning-extinction-and-morphine-analgesia","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/biv-03-effect-of-%ce%bc-opioid-receptor-knockout-on-fear-conditioning-extinction-and-morphine-analgesia\/","title":{"rendered":"BIV-03: Effect of \u03bc-Opioid Receptor Knockout on Fear Conditioning, Extinction, and Morphine-Analgesia"},"content":{"rendered":"\n

Our understanding of the biological mechanisms underlying fear, trauma, and the development of related disorders like post-traumatic stress disorder (PTSD) are incomplete.\u00a0 Thus, the search for players in the game continues.\u00a0 Among the brain regions identified as exhibiting maladaptive changes\/activity in PTSD, the amygdala appears to serve an important role in the acquisition and expression of fear.\u00a0 Within the amygdala, the basolateral nucleus (BLA) integrates sensory information, with the central nucleus (CeA) helping to initiate\/regulate a subsequent behavioral response.\u00a0 Between the BLA and CeA lie a subset of GABAergic neurons known as the intercalated interneurons (ITCs).\u00a0 They serve to modulate signaling, primarily from the BLA to the CeA.\u00a0 The result of this is inhibition of the CeA via ITC activation by the BLA.\u00a0 These ITC neurons also express the inhibitory mu-opioid receptor (MOR) very densely, and thus are sensitive to both the endorphins\/enkephalins and exogenous opioids.\u00a0 Importantly, ITCs appear to play a significant part in fear extinction.\u00a0 In this experiment, we sought to determine the role of ITC MORs in fear acquisition and extinction.\u00a0 To do this, we utilized a Cre expression (Cre- or Cre+) dependent knockout (KO) of Oprm1 from FoxP2-expressing neurons (e.g., ITCs).\u00a0 Our results show significant interactions between both genotype and day as well as sex and day.\u00a0 The effects are such that Cre+ (KO) mice extinguish fear at a slower rate than Cre-, and female mice extinguish fear slower than male mice.\u00a0 These results imply that ITC MORs are involved in fear extinction.\u00a0 Additionally, we conducted a control experiment using a morphine analgesia test on the hotplate to confirm the specificity of our MOR KO in Cre+ mice.\u00a0 There were no significant differences in latencies across groups, demonstrating no effect of MOR KO on morphine analgesia.<\/p>\n\n\n\n

Presenter(s): Henry Beckett, Psychology and Neuroscience Major
Advisor(s): Jennifer Quinn, Department of Psychology
Amanda Reichert, Department of Psychology
Jacob Dowell, Department of Psychology
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Our understanding of the biological mechanisms underlying fear, trauma, and the development of related disorders like post-traumatic stress disorder (PTSD) are incomplete.\u00a0 Thus, the search for players in the game continues.\u00a0 Among the brain regions identified as exhibiting maladaptive changes\/activity in PTSD, the amygdala appears to serve an important role in the acquisition and expression […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[1299,1,52,1321],"tags":[1519,1420,98,661],"class_list":["post-8488","post","type-post","status-publish","format-standard","hentry","category-2023-talks","category-projects","category-psychology","category-session-iv-bystrom","tag-amygdala","tag-fear-conditioning","tag-neuroscience","tag-psychology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-2cU","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/8488","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=8488"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/8488\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=8488"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=8488"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=8488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}