{"id":8415,"date":"2023-04-18T21:04:22","date_gmt":"2023-04-19T01:04:22","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=8415"},"modified":"2023-04-18T21:07:12","modified_gmt":"2023-04-19T01:07:12","slug":"csiv-05","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/csiv-05\/","title":{"rendered":"CSIV-05: Role of Hypoxia on Chick Retinal Pigment Epithelium Reprogramming"},"content":{"rendered":"\n

The retina is one of the most important tissues since an injury to the retina can lead to irreparable visual loss. Therefore, studying the process of retinal regeneration is important for understanding how the retina can be healed after an injury. The embryonic chick has been identified as a model for retinal regeneration, as until day 4 of embryonic development it is capable of regenerating the retina when treated with certain factors, such as FGF2. FGF2 induces the regeneration of the retina by reprogramming the retinal pigment epithelium (RPE), a tissue found behind the retina. However, the molecular mechanisms which allow FGF2 to induce RPE reprogramming are not completely understood. Several genes necessary for RPE reprogramming are also required during cellular reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) and are regulated by the hypoxia-inducing factor 1a (HIF1a), a transcription factor. We hypothesize that HIF1a activity is also required for RPE reprogramming in the context of retina regeneration. In order to test this hypothesis, we used RPE explants collected from 4-day-old chicken embryos and cultured them in the presence and absence of FGF2 while also inducing or inhibiting HIF1a activity by adding the small molecules DMOG and Echinomycin. We observed retinal growth in the various treatments, and then validated the presence of HIF1a via a Western Blot. To characterize the effects of HIF1a on RPE reprogramming, we determined the expression of genes related to reprogramming such as retina markers, RPE markers, and genes related to HIF1a activity. We observed differential expression of some genes related to RPE cell identity as well as retinal cell identity, suggesting that HIF1a plays a partial role in the reprogramming process. Altogether, these findings will help contribute to the understanding of retinal regeneration and directly relates to my interest in becoming a physician.<\/p>\n\n\n\n

Presenter(s): Harshavardhan Sanaka, Biology and Premedical Studies Major
Advisor(s): Katia Del Rio-Tsonis, Department of Biology
Jos\u00e9 Ra\u00fal P\u00e9rez-Estrada, Department of Biology<\/p>\n\n\n\n

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The retina is one of the most important tissues since an injury to the retina can lead to irreparable visual loss. Therefore, studying the process of retinal regeneration is important for understanding how the retina can be healed after an injury. The embryonic chick has been identified as a model for retinal regeneration, as until […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[1299,1,24,1323],"tags":[878,1027,89],"class_list":["post-8415","post","type-post","status-publish","format-standard","hentry","category-2023-talks","category-projects","category-biology","category-session-iv-caroline-scott","tag-biology","tag-retinal-regeneration","tag-stem"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-2bJ","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/8415","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=8415"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/8415\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=8415"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=8415"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=8415"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}