{"id":4465,"date":"2021-04-19T17:56:43","date_gmt":"2021-04-19T21:56:43","guid":{"rendered":"http:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=4465"},"modified":"2021-04-20T11:47:54","modified_gmt":"2021-04-20T15:47:54","slug":"a38-p-determining-the-role-nkx6-1-plays-in-lens-fiber-cell-differentiation-in-relation-to-pax6-expression","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/a38-p-determining-the-role-nkx6-1-plays-in-lens-fiber-cell-differentiation-in-relation-to-pax6-expression\/","title":{"rendered":"A38-P: Determining the Role NKX6-1 Plays in Lens Fiber Cell Differentiation in Relation to PAX6 Expression"},"content":{"rendered":"\n

The role of NKX6-1 in the lens and its relationship with PAX6 in lens development is not well understood. However, data from other studies suggest that there is an antagonistic relationship between PAX6 and NKX6-1 in the development of the neural tube and the pancreas. Therefore, we hypothesize that at least part of the development of the lens PAX 6 and NKX6-1 display an antagonistic relationship which results in NKX6-1 repressing Pax6. Specifically, we aim to determining if the PAX6 expression pattern changes in NKX6-1lox\/lox Cre+ mice lenses and how both the overexpression and lack of NKX6-1 in mouse lenses affects the levels of expression by PAX6 and other genes associated with PAX6 in the lens. To understand this relationship we looked at a H&E Stain of NKX6-1lox\/lox Cre+ mice compared to wild-type mice to determine if lens structure had changed. We found there was no change to the lens structure. In the future, we will perform an immunochemistry of NKX6-1lox\/lox Cre+ mice compared to wild-type mice to determine if the PAX6 expression pattern in the lenses changed. We will also compare the transcript expression of PAX6 and other PAX6 associated genes in NKX6-1lox\/lox Cre+ , NKX6-1 overexpressed, and wild type lenses by extracting RNA and completing a qPCR to compare expression levels. We expect to see PAX6 expression increase in the fiber cells of the lens in the NKX6-1lox\/lox Cre+ . It is also expected that there will be a decrease in the expression of PAX6 and PAX6 associated genes that are up-regulated in NKX6-1 overexpressed lenses compared to wild-type lenses. The opposite effect is observed in the NKX6-1lox\/lox Cre+mice compared to wild-type lenses. I plan to apply the knowledge I have gained from this project towards my future work in the medical field. <\/p>\n\n\n\n

Author<\/strong>: Dana Kapcio<\/p>\n\n\n\n

Faculty Advisor<\/strong>: Michael Robinson, Biology <\/p>\n\n\n\n

Graduate Student Advisor<\/strong>: Tycho Jaquish, Biology <\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

The role of NKX6-1 in the lens and its relationship with PAX6 in lens development is not well understood. However, data from other studies suggest that there is an antagonistic relationship between PAX6 and NKX6-1 in the development of the neural tube and the pancreas. Therefore, we hypothesize that at least part of the development […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[502,506,1,24],"tags":[842],"class_list":["post-4465","post","type-post","status-publish","format-standard","hentry","category-2021-posters","category-a35-45","category-projects","category-biology","tag-lens-development"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-1a1","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/4465","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=4465"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/4465\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=4465"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=4465"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=4465"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}