{"id":1229,"date":"2020-04-24T17:16:26","date_gmt":"2020-04-24T21:16:26","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=1229"},"modified":"2020-04-27T15:12:18","modified_gmt":"2020-04-27T19:12:18","slug":"updated-poster-investigation-of-effects-of-salicylic-acid-on-mitochondrial-tatc-expression-in-arabidopsis-thaliana","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/updated-poster-investigation-of-effects-of-salicylic-acid-on-mitochondrial-tatc-expression-in-arabidopsis-thaliana\/","title":{"rendered":"A12: Investigation of Effects of Salicylic Acid on Mitochondrial TatC Expression in Arabidopsis thaliana"},"content":{"rendered":"<p>The Twin Arginine Translocation pathway (TAT) is a protein pathway that is fueled only by the proton motive force, can transport fully folded proteins, and is present in bacteria, archaea and eukaryotic organelles such as the chloroplast and mitochondria. It is well known as to the function of this pathway in bacteria and chloroplasts, though it is unknown as to the function of the mitochondrial Tat pathway. In this study, we subjected Arabidopsis thaliana plants to various concentrations of salicylic acid and did a time course study assessing the amount of mtTatC protein present using immunoblotting. We hope to see an increase in mtTatC amount over time and with increasing salicylic acid concentration. Cellular transport is very important for any organism\u2019s cellular functioning and thus this research aids in the understanding of cellular processes that are vital for life.<\/p>\n<p><strong>Authors:<\/strong> Brooke Buckingham<\/p>\n<p><strong>Faculty Advisor:<\/strong> Carole Dabney-Smith, Department of Biochemistry<\/p>\n<p><strong>Graduate Student Advisor:<\/strong> Katie Eudy, Department of Biochemistry<\/p>\n<p><a href=\"https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?ssl=1\" rel=\"attachment wp-att-1230\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"1230\" data-permalink=\"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/updated-poster-investigation-of-effects-of-salicylic-acid-on-mitochondrial-tatc-expression-in-arabidopsis-thaliana\/a12brooke\/\" data-orig-file=\"https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?fit=1440%2C1080&amp;ssl=1\" data-orig-size=\"1440,1080\" data-comments-opened=\"0\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"A12Brooke\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?fit=770%2C578&amp;ssl=1\" class=\"alignleft wp-image-1230 size-large\" title=\"a12brooke-png\" src=\"https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?resize=770%2C578&#038;ssl=1\" alt=\"\" width=\"770\" height=\"578\" srcset=\"https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?resize=600%2C450&amp;ssl=1 600w, https:\/\/i0.wp.com\/sites.miamioh.edu\/undergraduate-research-forum\/files\/2020\/04\/A12Brooke.png?w=1440&amp;ssl=1 1440w\" sizes=\"auto, (max-width: 770px) 100vw, 770px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Twin Arginine Translocation pathway (TAT) is a protein pathway that is fueled only by the proton motive force, can transport fully folded proteins, and is present in bacteria, archaea and eukaryotic organelles such as the chloroplast and mitochondria. It is well known as to the function of this pathway in bacteria and chloroplasts, though [&hellip;]<\/p>\n","protected":false},"author":2899,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[10,27],"tags":[429,89],"class_list":["post-1229","post","type-post","status-publish","format-standard","hentry","category-2020-posters","category-chemistry-biochemistry","tag-proteins","tag-stem"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-jP","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/1229","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/2899"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=1229"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/1229\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=1229"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=1229"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=1229"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}