{"id":10181,"date":"2024-06-16T12:20:09","date_gmt":"2024-06-16T16:20:09","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=10181"},"modified":"2024-10-24T14:42:15","modified_gmt":"2024-10-24T18:42:15","slug":"a43-striatal-cholinergic-interneurons-influence-ethanol-drinking","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/a43-striatal-cholinergic-interneurons-influence-ethanol-drinking\/","title":{"rendered":"A43: Striatal Cholinergic Interneurons Influence Ethanol Drinking"},"content":{"rendered":"\n

Heavy alcohol use and binge drinking are important contributors to alcohol use disorder (AUD). The endogenous opioid system has been implicated in alcohol consumption and preference in both humans and animals. The mu opioid receptor (MOR) is expressed on multiple cells in the striatum, however little is known about the contributions of specific MOR populations to alcohol drinking behaviors. The current study used mice with a genetic deletion of MOR in cholinergic cells (ChAT-Cre\/Oprm1fl\/fl) to examine the role of MORs expressed in cholinergic interneurons (CINs) in home cage self-administration paradigms. Male and female ChAT-Cre\/Oprm1fl\/fl mice were generated and heterozygous Cre+ (knockout) and Cre- (control) mice were tested for alcohol, nicotine, and sucrose consumption. In Experiment 1, binge-like and quinine-resistant drinking were tested using 15% ethanol (EtOH) in a two-bottle, limited-access Drinking in the Dark paradigm. Experiment 2 involved a two\u2013week continuous access home cage drinking paradigm with sucrose for the first week and quinine the second week. Deleting MORs in cholinergic cells did not alter consumption of EtOH in Experiment 1, but it did result in greater consumption of quinine-adulterated EtOH in male Cre+ mice (vs. Cre-). In Experiment 2, Cre+ female mice displayed increased consumption and preference for the sucrose solution. Additionally, deletion of MORs did not alter baseline taste aversion for quinine. These data suggest that cholinergic MORs may have a sex-dependent effect on aversion resistant EtOH drinking in addition to sucrose consumption and preference.<\/p>\n\n\n\n

Author(s): Cambria R. Beane and Anna K. Radke, Ph.D<\/p>\n\n\n\n

Advisor(s): Anna K. Radke, Psychology Department<\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

Heavy alcohol use and binge drinking are important contributors to alcohol use disorder (AUD). The endogenous opioid system has been implicated in alcohol consumption and preference in both humans and animals. The mu opioid receptor (MOR) is expressed on multiple cells in the striatum, however little is known about the contributions of specific MOR populations […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[1589,1612,1,52],"tags":[331,1889,1888,98,661,89],"class_list":["post-10181","post","type-post","status-publish","format-standard","hentry","category-2024-posters","category-a41-50-2024-posters","category-projects","category-psychology","tag-addiction","tag-cholinergic-interneurons","tag-ethanol-drinking","tag-neuroscience","tag-psychology","tag-stem"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-2Ed","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/10181","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=10181"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/10181\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=10181"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=10181"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=10181"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}