{"id":10097,"date":"2024-06-14T20:24:58","date_gmt":"2024-06-15T00:24:58","guid":{"rendered":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/?p=10097"},"modified":"2024-10-24T14:57:50","modified_gmt":"2024-10-24T18:57:50","slug":"a54-differences-in-dna-methylation-patterns-of-the-atp1a4-gene-between-spermatogenic-and-somatic-cell-lines","status":"publish","type":"post","link":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/a54-differences-in-dna-methylation-patterns-of-the-atp1a4-gene-between-spermatogenic-and-somatic-cell-lines\/","title":{"rendered":"A54: Differences in DNA-Methylation Patterns of the ATP1A4 Gene Between Spermatogenic and Somatic Cell Lines"},"content":{"rendered":"\n

The Na+\/K+-ATPase alpha4 (ATP1A4 gene) ion transporter is an essential protein for sperm cells, as it helps establish an ion gradient used by secondary Na\/H exchangers, ultimately directing the intracellular pH of sperm. As this gene has been found significant in spermatogenic cells, previous literature points to differences in DNA methylation patterns of the ATP1A4 gene in spermatogonial cells versus somatic cells that may contribute to its tissue-specific expression. This project aims to investigate and compare the methylation patterns of this gene across human, mouse, and rat cell culture systems, as well as identify the effects of modifying the DNA methylation patterns. We hypothesized that the methylation patterns will differ between the spermatogenic and somatic cells, and predicted that in spermatogenic cells, the ATP1A4 regulatory elements (namely CpG islands) would be less methylated, correlating to higher protein expression. To test this hypothesis, bisulfite sequencing was executed on DNA from the MK4 (representing a non) and GC1-spg cell (representing a spermatogenic cell) lines (both derived from mice). The CRISPR\/dCas9 system will be employed to investigate effects of modifying methylation patterns on gene expression. These same methods will be further applied to spermatogenic and somatic cell culture systems in humans and rats. Data mining and in silico results from this study found that similar regions of the mouse, rat, and human ATP1A4 gene contain CpG islands\/CpG-containing sites. Bisulfite sequencing of the MK4 and GC1-spg cell lines revealed differences in the methylation of these CpG-containing sites when comparing spermatogenic and somatic cells. Results of this study add to the growing foundation related to epigenetic research and can be applied to fields regarding epigenetic alteration and male contraceptives that could potentially render sperm immotile and nonfunctional.<\/p>\n\n\n\n

Author(s): Kris Hackiewicz, Odunayo Ayodele & Paul F. James<\/p>\n\n\n\n

Advisor(s): Paul F. James, Biology<\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

The Na+\/K+-ATPase alpha4 (ATP1A4 gene) ion transporter is an essential protein for sperm cells, as it helps establish an ion gradient used by secondary Na\/H exchangers, ultimately directing the intracellular pH of sperm. As this gene has been found significant in spermatogenic cells, previous literature points to differences in DNA methylation patterns of the ATP1A4 […]<\/p>\n","protected":false},"author":301,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[1589,1613,1,24],"tags":[878,1868,1869,89],"class_list":["post-10097","post","type-post","status-publish","format-standard","hentry","category-2024-posters","category-a51-60-2024-posters","category-projects","category-biology","tag-biology","tag-epigenetics","tag-molecular-physiology","tag-stem"],"aioseo_notices":[],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/pbRpLx-2CR","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/10097","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/users\/301"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/comments?post=10097"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/posts\/10097\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/media?parent=10097"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/categories?post=10097"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.miamioh.edu\/undergraduate-research-forum\/wp-json\/wp\/v2\/tags?post=10097"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}