{"id":636,"date":"2018-02-07T13:32:22","date_gmt":"2018-02-07T18:32:22","guid":{"rendered":"http:\/\/sites.miamioh.edu\/tomoyasulab\/?page_id=636"},"modified":"2018-09-05T12:25:17","modified_gmt":"2018-09-05T16:25:17","slug":"plasmids","status":"publish","type":"page","link":"https:\/\/sites.miamioh.edu\/tomoyasulab\/plasmids\/","title":{"rendered":"Plasmids"},"content":{"rendered":"

Insect transformation plasmids<\/span><\/h1>\n

Here is the collection of our piggyBac-based insect transformation plasmids<\/p>\n

piggyGUM<\/strong> \r\n\r\n\"\"<\/a>A GATEWAY compatible reporter construct for evaluating enhancer activity. DSCP (Drosophila Synthesis Core promoter) with mCherry.\r\n\r\nDownload annotated sequence (genbank format) here\r\n <\/a><\/span>\r\n\r\nreference:\u00a0Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum<\/em> \r\n(Lai et al. Development 2018<\/a>)<\/pre>\n
 <\/p>\n
piggyGHR\r\n<\/strong>\r\n\"\"<\/a>A GATEWAY compatible reporter construct for evaluating enhancer activity. Tc-hsp68 minimal promoter with dsRed. This construct worked in Drosophila, but did not work in Tribolium (at least in the wing tissue).\r\n\r\nDownload annotated sequence (genbank format) here<\/a>\r\n\r\nreference:\u00a0Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum<\/em> \r\n(Lai et al. Development 2018<\/a>)<\/pre>\n
piggyAct5cR\r\n<\/strong>\r\n\"\"<\/a>\r\n\r\nTribolium act5C promoter (1kb upstream from the start codon, including 5'UTR) driving dsRed. The native polyA (1kb downstream from the stop codon including 3UTR and polyA) is used. This construct drives strong ubiquitous expression in Tribolium, but it does not drive any expression in Drosophila.\r\n\r\nDownload annotated sequence (genbank format) here<\/a>\r\n\r\nreference:\u00a0Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum<\/em> \r\n(Lai et al. Development 2018<\/a>)<\/pre>\n
piggyNubProR\r\n<\/strong>\r\n\"\"<\/a>\r\n\r\nTribolium nubbin promoter (2kb upstream from the start codon, including 5'UTR) driving dsRed. The native polyA (2kb downstream from the stop codon including 3UTR and polyA) is used. This construct fails to drive dsRed expression in Tribolium.\r\n\r\nDownload annotated sequence (genbank format) here<\/a>\r\n\r\nreference:\u00a0Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum<\/em> \r\n(Lai et al. Development 2018<\/a>)<\/pre>\n
More transformation plasmids coming soon!<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"
Insect transformation plasmids Here is the collection of our piggyBac-based insect transformation plasmids piggyGUM A GATEWAY compatible reporter construct for evaluating enhancer activity. DSCP (Drosophila Synthesis Core promoter) with mCherry. Download annotated sequence (genbank format) here reference:\u00a0Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum (Lai et al. Development 2018)   piggyGHR […]<\/p>\n","protected":false},"author":1595,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"class_list":["post-636","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/pages\/636","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/users\/1595"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/comments?post=636"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/pages\/636\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/tomoyasulab\/wp-json\/wp\/v2\/media?parent=636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}