{"id":165,"date":"2017-02-21T09:30:02","date_gmt":"2017-02-21T14:30:02","guid":{"rendered":"http:\/\/sites.miamioh.edu\/cricket-lab\/?page_id=165"},"modified":"2017-08-08T11:51:23","modified_gmt":"2017-08-08T15:51:23","slug":"imaging-techniques","status":"publish","type":"page","link":"https:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/imaging-techniques\/","title":{"rendered":"Imaging Techniques"},"content":{"rendered":"<p>Our lab uses a variety of imaging techniques to visualize septate junctions in the gut and stem cell niche of the brain as well as the gross morphology of the tissue.<\/p>\n<p><span style=\"color: #333333\"><strong>Transmission Electron Microscopy<\/strong><\/span>: Images of the cricket gut<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-33\" src=\"http:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/cell-cell-pics-300x257.png\" alt=\"\" width=\"300\" height=\"257\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/cell-cell-pics-300x257.png 300w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/cell-cell-pics-768x658.png 768w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/cell-cell-pics.png 800w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-36\" src=\"http:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/peritrophic-membrane-and-bacteria-261x300.jpg\" alt=\"\" width=\"223\" height=\"243\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-397\" src=\"http:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR-169x300.jpg\" alt=\"\" width=\"158\" height=\"281\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR-169x300.jpg 169w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR-768x1367.jpg 768w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR-575x1024.jpg 575w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR-197x350.jpg 197w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR-84x150.jpg 84w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/IMG_20170321_150825687_HDR.jpg 1456w\" sizes=\"auto, (max-width: 158px) 100vw, 158px\" \/><\/p>\n<p><span style=\"color: #993300\"><strong>Confocal<\/strong><\/span>: We use differential interference contrast (DIC) and florescent microscopy<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-38\" src=\"http:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/brain-300x241.png\" alt=\"\" width=\"300\" height=\"241\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/brain-300x241.png 300w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/brain.png 524w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p><span style=\"color: #993366\"><strong>Light Microscopy<\/strong><\/span>:\u00a0Hematoxylin and eosin stain<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-29\" src=\"http:\/\/sites.miamioh.edu\/cricket-lab\/files\/2016\/11\/Img-04-300x199.jpg\" alt=\"\" width=\"300\" height=\"199\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2016\/11\/Img-04-300x199.jpg 300w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2016\/11\/Img-04-768x510.jpg 768w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2016\/11\/Img-04-1024x680.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-182\" src=\"http:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4-300x300.jpg\" alt=\"\" width=\"200\" height=\"201\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4-300x300.jpg 300w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4-150x150.jpg 150w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4-768x768.jpg 768w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4-1024x1024.jpg 1024w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4-350x350.jpg 350w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Lach-Project-CTRL-26-7-01-4b4.jpg 1200w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our lab uses a variety of imaging techniques to visualize septate junctions in the gut and stem cell niche of the brain as well as the gross morphology of the tissue. Transmission Electron Microscopy: Images of the cricket gut \u00a0 Confocal: We use differential interference contrast (DIC) and florescent microscopy \u2026 <a class=\"continue-reading-link\" href=\"https:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/imaging-techniques\/\"> Continue reading<\/a><\/p>\n","protected":false},"author":1974,"featured_media":0,"parent":156,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"class_list":["post-165","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/165","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/users\/1974"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/comments?post=165"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/165\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/156"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/media?parent=165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}