{"id":158,"date":"2017-02-21T09:25:29","date_gmt":"2017-02-21T14:25:29","guid":{"rendered":"http:\/\/sites.miamioh.edu\/cricket-lab\/?page_id=158"},"modified":"2017-11-21T10:01:21","modified_gmt":"2017-11-21T15:01:21","slug":"behavior","status":"publish","type":"page","link":"https:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/behavior\/","title":{"rendered":"Behavior Assays"},"content":{"rendered":"<p>In order to assess the effect cold stress has on our model organism, we perform fighting and mating behavioral assays. Changes in fight\u00a0aggressiveness, dominance or ability to mate suggest alterations to the neurochemistry in the brain that affect motivation or debilitating physical changes.<\/p>\n<p><strong><span style=\"color: #bf750d\">Fighting Behavior<\/span><\/strong><\/p>\n<p><span style=\"color: #333333\">For this assay, we \u00a0separate male crickets by an opaque barrier with a clear plexiglass arena and allow them to acclimate to their new environment. After 15 minutes, we remove the barrier and allow interaction. Male crickets will fight for social dominance and the winner will sing\u00a0a victory song while the loser flees. The aggressiveness of the fight is\u00a0scored on a scale of 0-6.<\/span><\/p>\n\n\t\t<style>\n\t\t\t#gallery-1 {\n\t\t\t\tmargin: auto;\n\t\t\t}\n\t\t\t#gallery-1 .gallery-item {\n\t\t\t\tfloat: left;\n\t\t\t\tmargin-top: 10px;\n\t\t\t\ttext-align: center;\n\t\t\t\twidth: 33%;\n\t\t\t}\n\t\t\t#gallery-1 img {\n\t\t\t\tborder: 2px solid #cfcfcf;\n\t\t\t}\n\t\t\t#gallery-1 .gallery-caption {\n\t\t\t\tmargin-left: 0;\n\t\t\t}\n\t\t\t\/* see gallery_shortcode() in wp-includes\/media.php *\/\n\t\t<\/style>\n\t\t<div id='gallery-1' class='gallery galleryid-158 gallery-columns-3 gallery-size-large'><dl class='gallery-item'>\n\t\t\t<dt class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/behavior\/fighitng-collage\/'><img loading=\"lazy\" decoding=\"async\" width=\"920\" height=\"573\" src=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage-1024x638.jpg\" class=\"attachment-large size-large\" alt=\"\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage-1024x638.jpg 1024w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage-300x187.jpg 300w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage-768x478.jpg 768w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage-562x350.jpg 562w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage-150x93.jpg 150w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Fighitng-collage.jpg 1680w\" sizes=\"auto, (max-width: 920px) 100vw, 920px\" \/><\/a>\n\t\t\t<\/dt><\/dl>\n\t\t\t<br style='clear: both' \/>\n\t\t<\/div>\n\n<p style=\"text-align: center\"><a href=\"https:\/\/youtu.be\/YYAFK9IFDfU\"><strong>Fighting Video<\/strong><\/a><\/p>\n<p><span style=\"color: #bf750d\"><strong>Mating Behavior<\/strong><\/span><br \/>\nMales and females are again separated by a barrier which is lifted after 15 minutes. The pair is allotted 10 minutes to mate. During mating, the female mounts on the back of the male. The male threads a sperm filled sac called a spermatophore onto the female. The sperm then swims up into the female&#8217;s spermatheca where they are stored until the female lays her eggs. The most common reason for failure to mate after cold stress is lack of a mature male spermatophore.<\/p>\n\n\t\t<style>\n\t\t\t#gallery-2 {\n\t\t\t\tmargin: auto;\n\t\t\t}\n\t\t\t#gallery-2 .gallery-item {\n\t\t\t\tfloat: left;\n\t\t\t\tmargin-top: 10px;\n\t\t\t\ttext-align: center;\n\t\t\t\twidth: 33%;\n\t\t\t}\n\t\t\t#gallery-2 img {\n\t\t\t\tborder: 2px solid #cfcfcf;\n\t\t\t}\n\t\t\t#gallery-2 .gallery-caption {\n\t\t\t\tmargin-left: 0;\n\t\t\t}\n\t\t\t\/* see gallery_shortcode() in wp-includes\/media.php *\/\n\t\t<\/style>\n\t\t<div id='gallery-2' class='gallery galleryid-158 gallery-columns-3 gallery-size-large'><dl class='gallery-item'>\n\t\t\t<dt class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/behavior\/collage-of-mating\/'><img loading=\"lazy\" decoding=\"async\" width=\"920\" height=\"574\" src=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating-1024x639.jpg\" class=\"attachment-large size-large\" alt=\"\" srcset=\"https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating-1024x639.jpg 1024w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating-300x187.jpg 300w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating-768x479.jpg 768w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating-561x350.jpg 561w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating-150x94.jpg 150w, https:\/\/sites.miamioh.edu\/cricket-lab\/files\/2017\/02\/Collage-of-Mating.jpg 1679w\" sizes=\"auto, (max-width: 920px) 100vw, 920px\" \/><\/a>\n\t\t\t<\/dt><\/dl>\n\t\t\t<br style='clear: both' \/>\n\t\t<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>In order to assess the effect cold stress has on our model organism, we perform fighting and mating behavioral assays. Changes in fight\u00a0aggressiveness, dominance or ability to mate suggest alterations to the neurochemistry in the brain that affect motivation or debilitating physical changes. Fighting Behavior For this assay, we \u00a0separate \u2026 <a class=\"continue-reading-link\" href=\"https:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/behavior\/\"> Continue reading<\/a><\/p>\n","protected":false},"author":1974,"featured_media":0,"parent":156,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"class_list":["post-158","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/158","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/users\/1974"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/comments?post=158"}],"version-history":[{"count":0,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/158\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/156"}],"wp:attachment":[{"href":"https:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/media?parent=158"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}