{"id":168,"date":"2017-02-21T09:31:02","date_gmt":"2017-02-21T14:31:02","guid":{"rendered":"http:\/\/sites.miamioh.edu\/cricket-lab\/?page_id=168"},"modified":"2017-11-21T09:42:38","modified_gmt":"2017-11-21T14:42:38","slug":"molecular-assays","status":"publish","type":"page","link":"http:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/molecular-assays\/","title":{"rendered":"Molecular Assays"},"content":{"rendered":"<p><strong>RNA interference<\/strong><\/p>\n<p>Systemic RNAi refers to the spread of gene-silencing effects from cell to cell throughout the entire organism.\u00a0 This process is initiated when dsRNA is administered to the organism and taken up by the cells.\u00a0 Upon entry into the cell, dsRNA processing, and the targeting and degradation of complementary mRNA occur.\u00a0 This technique allows us to create a loss-of-function phenotype at the desired stage of development by simply injecting the cricket with double-stranded RNA.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>RNA interference Systemic RNAi refers to the spread of gene-silencing effects from cell to cell throughout the entire organism.\u00a0 This process is initiated when dsRNA is administered to the organism and taken up by the cells.\u00a0 Upon entry into the cell, dsRNA processing, and the targeting and degradation of complementary \u2026 <a class=\"continue-reading-link\" href=\"http:\/\/sites.miamioh.edu\/cricket-lab\/techniques\/molecular-assays\/\"> Continue reading<\/a><\/p>\n","protected":false},"author":1974,"featured_media":0,"parent":156,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"class_list":["post-168","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/168","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/users\/1974"}],"replies":[{"embeddable":true,"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/comments?post=168"}],"version-history":[{"count":0,"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/168\/revisions"}],"up":[{"embeddable":true,"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/pages\/156"}],"wp:attachment":[{"href":"http:\/\/sites.miamioh.edu\/cricket-lab\/wp-json\/wp\/v2\/media?parent=168"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}